The aim of this study was to investigate relationship between the sensation seeking trait and occupational choice, as well as to check the existence of differences between girls and boys in their occupational choices in relation to the sensation seeking traits. The data was collected among 292 pupils aged 17 – 19 attending grade 4 in three grammar schools from Osijek and Beli Manastir. Pupils have filled in two surveys – Holland's SelfDirected Search and Zuckerman Sensation Seeking Scale. The results of the regression analyses showed that the measures of sensation seeking predict all professional interests, but not always in expected direction. The importance of diversity of arithmetic mean was tested between girls and boys for measuring sensation and professional interests. The results show that pupils have considerably better results in total related to sensation seeking trait and all subscales except for the subscale of experience seeking. Boys showed stronger realistic, enterprising and conventional interests and girls showed stronger artistic and social ones while they do not show any significant differences in investigative interests. Regression analyses have been made with measures for sensation seeking as predictors of occupational choices in regard of gender. Measures for sensation seeking are significant predictors of all professional interests except for realistic ones for boys while there are significant predictors of realistic, investigative, artistic and enterprising interests for girls.