Few studies have analyzed the relationship between the Dark Triad and reproductive strategies, considering preferences for specific types of relationships. Therefore, the aim of this research was to investigate the relationship between Machiavellianism, narcissism, and psychopathy, as well as reproductive strategies and preferences for certain types of relationships in men and women. The research was conducted online on a sample of 387 participants who filled out Questionnaire of socio-demographic data, Short Dark Triad, and Revised Sociosexual Orientation Inventory. Also, the participants performed budget allocation task with the aim of examining the preference of certain types of relationships. The obtained results indicate that there is a positive correlation between the Dark Triad and a short-term reproductive strategy. Furthermore, the results of the correlation analysis showed that women high in Machiavellianism and psychopathy, as well as men high in psychopathy and narcissism, prefer short-term relationships (one-night stand, booty-call and friends-with-benefits). Men high in narcissism prefer short-term relationships (booty-call and one-night stand). Finally, the mediation analysis showed that psychopathy mediates the sex difference in preferences for booty-call relationship, and partially mediates sex difference in preference for one-night stands. In addition, a Machiavellianism partially mediates sex difference in preference for friends-with-benefits relationship. The results showed that both psychopathy and Machiavellianism partially mediate sex difference in preference for serious romantic relationship. This research has deepened the understanding of the complex interactions between dark personality traits and reproductive strategies. Further research is needed to generalize the findings, thus contributing to existing knowledge regarding the mechanisms underlying partner selection and retention.