The aim of this study was to investigate the relationship of public stigma associated with receiving professional psychological help, psychopathological symptoms and attitudes towards seeking professional psychological help, and to examine gender differences in the variables above. The aim was also to determine the role of public stigma associated with seeking professional psychological help and psychopathological symptoms in predicting attitudes towards seeking professional psychological help. The sample consisted of 494 students from various universities across the Republic of Croatia. Participants completed the questionnaire The Attitudes Toward Seeking Professional Psychological Help-Short Form, ATSPPH-SF (Fischer, & Farina, 1995), Stigma scale for Receiving Professional Assistance, SSRPH (Vogel, Wester, Wei, & Boysen, 2005) and the Brief Symptom Inventory, BSI (Derogatis, & Melisaratos, 1983). The results show that there is a negative correlation between attitudes toward seeking professional psychological help and public stigma towards seeking professional psychological help, psychopathological symptoms and gender. Significant predictors of attitudes towards seeking professional psychological help are the public stigma associated with seeking professional psychological help and gender. Female gender and lower perceptions of public stigma towards seeking professional psychological help predict more positive attitudes about seeking professional psychological help. Also, there are differences between male and female participants in attitudes towards seeking professional psychological help, public stigma associated with seeking professional psychological help, and psychopathological symptoms. Young women express more psychopathological symptoms and a more positive attitude about seeking professional psychological help, and young men express higher perception of stigma towards seeking professional psychological help.